Ternary monolayers as DNA recognition interfaces for direct and sensitive electrochemical detection in untreated clinical samples
Autor(es) y otros:
Palabra(s) clave:
Dithiols
Clinical samples
Hybridization
DNA
Fecha de publicación:
Editorial:
Elsevier
Versión del editor:
Citación:
Descripción física:
Resumen:
Detection of specific DNA sequences in clinical samples is a key goal of studies on DNA biosensors and gene chips. Herein we present a highly sensitive electrochemical genosensor for direct measurements of specific DNA sequences in undiluted and untreated human serum and urine samples. Such genosensing relies on a new ternary interface involving hexanedithiol (HDT) co-immobilized with the thiolated capture probe (SHCP) on gold surfaces, followed by the incorporation of 6-mercapto-1-hexanol (MCH) as diluent...
Detection of specific DNA sequences in clinical samples is a key goal of studies on DNA biosensors and gene chips. Herein we present a highly sensitive electrochemical genosensor for direct measurements of specific DNA sequences in undiluted and untreated human serum and urine samples. Such genosensing relies on a new ternary interface involving hexanedithiol (HDT) co-immobilized with the thiolated capture probe (SHCP) on gold surfaces, followed by the incorporation of 6-mercapto-1-hexanol (MCH) as diluent...
Patrocinado por:
Financial support from the National Institutes of Health (Award U01 AI075565) is gratefully acknowledged. SC, MJLC and FK acknowledge fellowships from Programa Becas Complutense del Amo (2010–2011), University of Oviedo and Spanish Ministerio de Ciencia e Innovacion (PR 2009-0430), and The Scientific and Technical Council of Turkey (TUBITAK), respectively. EP and MB thank the Czech-Republic Ministry of Education, Youth and Sports(ME09038)
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