Simple bio-conjugation of polymer-coated quantum dots with antibodies for fluorescence-based immunoassays
Fecha de publicación:
2008
Versión del editor:
Citación:
Analyst, 133, p. 444-447 (2008); doi:10.1039/b802360n
Descripción física:
p. 444-447
Resumen:
A simple procedure for the bio-conjugation of amphiphilic polymer-coated quantum dots with antibodies is described. The formed bio-conjugates are purified from an excess of free antibodies and of free nanoparticles by size-exclusion HPLC and then characterized by fluorescence emission and MALDI-TOFMS. The applicability of the approach is demonstrated for aflatoxin B1 detection.
ISSN:
Identificador local:
584
DOI:
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