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Simple bio-conjugation of polymer-coated quantum dots with antibodies for fluorescence-based immunoassays

dc.contributor.authorFernández Fernández-Argüelles, María Teresa 
dc.contributor.authorCosta Fernández, José Manuel 
dc.contributor.authorPereiro García, María Rosario 
dc.contributor.authorSanz Medel, Alfredo 
dc.date.accessioned2013-01-30T10:20:09Z
dc.date.available2013-01-30T10:20:09Z
dc.date.issued2008
dc.identifier.citationAnalyst, 133, p. 444-447 (2008); doi:10.1039/b802360nspa
dc.identifier.issn0003-2654
dc.identifier.urihttp://hdl.handle.net/10651/10668
dc.description.abstractA simple procedure for the bio-conjugation of amphiphilic polymer-coated quantum dots with antibodies is described. The formed bio-conjugates are purified from an excess of free antibodies and of free nanoparticles by size-exclusion HPLC and then characterized by fluorescence emission and MALDI-TOFMS. The applicability of the approach is demonstrated for aflatoxin B1 detection.spa
dc.format.extentp. 444-447spa
dc.language.isoeng
dc.relation.ispartofAnalystspa
dc.sourceWOKspa
dc.titleSimple bio-conjugation of polymer-coated quantum dots with antibodies for fluorescence-based immunoassaysspa
dc.typejournal article
dc.identifier.local584spa
dc.identifier.doi10.1039/b802360n
dc.relation.publisherversionhttp://dx.doi.org/10.1039/b802360nspa


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