dc.contributor.author | Naughton, Catherine | |
dc.contributor.author | Huidobro Fernández, Covadonga | |
dc.contributor.author | Catacchio, Claudia R. | |
dc.contributor.author | Buckle, Adam | |
dc.contributor.author | Grimes, Graeme R. | |
dc.contributor.author | Nozawa, Ryu-Suke | |
dc.contributor.author | Purgato, Stefania | |
dc.contributor.author | Rocchi, Mariano | |
dc.contributor.author | Gilbert, Nick | |
dc.date.accessioned | 2024-01-31T07:08:24Z | |
dc.date.available | 2024-01-31T07:08:24Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Nature Communications, 13:5609 (2022); doi:10.1038/s41467-022-33426-2. | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://hdl.handle.net/10651/71073 | |
dc.description.abstract | Human centromeres appear as constrictions on mitotic chromosomes and form a platform for kinetochore assembly inmitosis. Biophysical experiments led to a suggestion that repetitive DNA at centromeric regions form a compact
scaffold necessary for function, but this was revised when neocentromeres were discovered on non-repetitive DNA. To test whether centromeres have a special chromatin structure we have analysed the architecture of a neocentromere. Centromere repositioning is accompanied by RNA polymerase II recruitment and active transcription to form a decompacted, negatively
supercoiled domain enriched in ‘open’ chromatin fibres. In contrast, centromerisation causes a spreading of repressive epigenetic marks to surrounding regions, delimited by H3K27me3 polycomb boundaries and divergent genes. This flanking domain is transcriptionally silent and partially remodelled to form ‘compact’ chromatin, similar to satellite-containing DNA
sequences, and exhibits genomic instability. We suggest transcription disrupts chromatin to provide a foundation for kinetochore formation whilst compact pericentromeric heterochromatin generates mechanical rigidity. | spa |
dc.description.sponsorship | Research Cancer UK, Medical Research Council. | spa |
dc.language.iso | eng | spa |
dc.relation.ispartof | Nature communications, 13, Article number: 5609 | spa |
dc.rights | © The Author(s) 2022 | |
dc.rights | CC Reconocimiento 4.0 Internacional | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | chromatin | spa |
dc.subject | transcription | spa |
dc.subject | centromere | spa |
dc.title | Human centromere repositioning activates transcription and opens chromatin fibre structure | spa |
dc.type | journal article | spa |
dc.identifier.doi | 10.1038/s41467-022-33426-2 | |
dc.relation.projectID | MR/J00913X/1 | spa |
dc.relation.projectID | MC_UU_00007/13 | spa |
dc.relation.publisherversion | https://doi.org/10.1038/s41467-022-33426-2. | |
dc.rights.accessRights | open access | spa |
dc.type.hasVersion | VoR | spa |