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A novel handheld fluorimeter for rapid detection of escherichia coli in drinking water

Autor(es) y otros:
Ferrero Martín, Francisco JavierAutoridad Uniovi; Valledor Llopis, MartaAutoridad Uniovi; Campo Rodríguez, Juan CarlosAutoridad Uniovi; Marín Fernández, LauraAutoridad Uniovi; Gutiérrez del Río Menéndez, IgnacioAutoridad Uniovi; Fernández Fernández, Javier; Lombó Brugos, FelipeAutoridad Uniovi; Cobián Fernández, Natalia; Olmos Fernández-Corugedo, FranciscoAutoridad Uniovi; Méndez Suárez, Irene
Palabra(s) clave:

Drinking water

Escherichia coli

Green fluorescent protein

Photomultiplier

Microcontroller

Fecha de publicación:
2016
Editorial:

IEEE

Versión del editor:
http://dx.doi.org/10.1109/JSEN.2016.2558649
Citación:
IEEE Sensors Journal, 16(13), p. 5136-5144 (2016); doi:10.1109/JSEN.2016.2558649
Resumen:

The microbiological quality of drinking water is a concern to consumers, water suppliers, regulators and public health authorities alike. Monitoring the microbiological quality of drinking water relies largely on examination of indicator bacteria such as coliforms like Escherichia coli. E. coli is widely used as an indicator of fecal pollution when monitoring the microbial quality of drinking water because it is abundant in all mammal feces and therefore is found in sewage and in natural waters contaminated with fecal matter, from human origin, wild animals or derived from agricultural activity. This paper describes the development of a novel handheld fluorimeter for rapid detection of E. coli in drinking water based on a specific cellular biomarker. The measurement system is based on a photomultiplier tube that captures the fluorescence signal produced by the cellular biomarker when it is excited by an ultraviolet LED. The cellular biomarker is also developed and it consists of a chimeric protein with a Green fluorescent protein in the N-terminal domain (GFP) and a specific amino acid sequence in the C-terminal domain (Colicin S4) that targets specifically the structure of the microorganism to be detected. The instrument is simple to use, lightweight, and can be powered by either an AC/DC power adapter or a rechargeable battery, making it an excellent choice for rapid detection of E. coli in drinking water in field studies and laboratory measurements

The microbiological quality of drinking water is a concern to consumers, water suppliers, regulators and public health authorities alike. Monitoring the microbiological quality of drinking water relies largely on examination of indicator bacteria such as coliforms like Escherichia coli. E. coli is widely used as an indicator of fecal pollution when monitoring the microbial quality of drinking water because it is abundant in all mammal feces and therefore is found in sewage and in natural waters contaminated with fecal matter, from human origin, wild animals or derived from agricultural activity. This paper describes the development of a novel handheld fluorimeter for rapid detection of E. coli in drinking water based on a specific cellular biomarker. The measurement system is based on a photomultiplier tube that captures the fluorescence signal produced by the cellular biomarker when it is excited by an ultraviolet LED. The cellular biomarker is also developed and it consists of a chimeric protein with a Green fluorescent protein in the N-terminal domain (GFP) and a specific amino acid sequence in the C-terminal domain (Colicin S4) that targets specifically the structure of the microorganism to be detected. The instrument is simple to use, lightweight, and can be powered by either an AC/DC power adapter or a rechargeable battery, making it an excellent choice for rapid detection of E. coli in drinking water in field studies and laboratory measurements

URI:
http://hdl.handle.net/10651/37263
ISSN:
1530-437X
DOI:
10.1109/JSEN.2016.2558649
Patrocinado por:

Financial support from IDEPA (Instituto de Desarrollo Económico del Principado de Asturias) and FICYT (Fundación para el Fomento en Asturias de la Investigación Científica Aplicada y la Tecnología) through the projects IE09-106 and IDE2012/000452 is gratefully acknowledged

Colecciones
  • Artículos [37532]
  • Ingeniería Eléctrica, Electrónica, de Comunicaciones y de Sistemas [1086]
  • Investigaciones y Documentos OpenAIRE [8365]
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