Development of a genosensor for peanut allergen ARA h 2 detection and its optimization by surface response methodology

Abstract

A new selective electrochemical genosensor has been developed for the detection of an 86-mer DNA peanut sequence encoding part of the allergen Arah2(conglutin-homologprotein).The method is base on a sandwich format,which presents two advantages:it permits shortening the capture probe and avoids labeling of the target.Screen-printed gold electrodes have been used as platformfor the immobilization of oligo nucleotides by the well-knownS–Aubond.Mixedself-assembled monolayers (SAM), including thiol-modified capture probe and mercaptohexanol,were prepared to a chieve an organized, homogeneous and not too compact SAM in which unspecific adsorption of the capture probe would be prevented...