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Simple bio-conjugation of polymer-coated quantum dots with antibodies for fluorescence-based immunoassays
dc.contributor.author | Fernández Fernández-Argüelles, María Teresa | |
dc.contributor.author | Costa Fernández, José Manuel | |
dc.contributor.author | Pereiro García, María Rosario | |
dc.contributor.author | Sanz Medel, Alfredo | |
dc.date.accessioned | 2013-01-30T10:20:09Z | |
dc.date.available | 2013-01-30T10:20:09Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Analyst, 133, p. 444-447 (2008); doi:10.1039/b802360n | spa |
dc.identifier.issn | 0003-2654 | |
dc.identifier.uri | http://hdl.handle.net/10651/10668 | |
dc.description.abstract | A simple procedure for the bio-conjugation of amphiphilic polymer-coated quantum dots with antibodies is described. The formed bio-conjugates are purified from an excess of free antibodies and of free nanoparticles by size-exclusion HPLC and then characterized by fluorescence emission and MALDI-TOFMS. The applicability of the approach is demonstrated for aflatoxin B1 detection. | spa |
dc.format.extent | p. 444-447 | spa |
dc.language.iso | eng | |
dc.relation.ispartof | Analyst | spa |
dc.source | WOK | spa |
dc.title | Simple bio-conjugation of polymer-coated quantum dots with antibodies for fluorescence-based immunoassays | spa |
dc.type | journal article | |
dc.identifier.local | 584 | spa |
dc.identifier.doi | 10.1039/b802360n | |
dc.relation.publisherversion | http://dx.doi.org/10.1039/b802360n | spa |
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