Quantum dot-based array for sensitive detection of Escherichia coli
Palabra(s) clave:
Bacteria . E. Coli . Antibody Array . Quantum Dot
Fecha de publicación:
2011
Versión del editor:
Citación:
Analytical and Bioanalytical Chemistry, 399(8), p. 1-8 (2011); doi:10.1007/s00216-010-4624-5
Descripción física:
p. 1-8
Resumen:
A fluorescent quantum dot-based antibody array, used in sandwich format, has been developed to detect Escherichia coli O157:H7. Numerous parameters such as solid support, optimal concentration of immunoreagents, blocking reagents, and assay time were optimized for array construction. Quantum dot-conjugated anti-IgG was used as the detecting system. The array allows the detection of E. coli O157:H7 at concentrations below 10 CFU mL−1 without sample enrichment, exhibiting an increase of three orders of magnitude in the limit of detection compared to ELISA. The interference caused by Gram (+) and Gram (−) bacteria was negligible at low concentrations of bacteria.
ISSN:
Identificador local:
20110078
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