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New integrated elemental and molecular strategies as a diagnostic tool for the quality of water soluble quantum dots and their bioconjugates

Author:
Trapiella Alfonso, LauraUniovi authority; Montoro Bustos, Antonio RafaelUniovi authority; Ruiz Encinar, JorgeUniovi authority; Costa Fernández, José ManuelUniovi authority; Pereiro García, María RosarioUniovi authority; Sanz Medel, AlfredoUniovi authority
Publication date:
2011
Publisher version:
http://dx.doi.org/10.1039/c0nr00822b
Citación:
Nanoscale, 3(3), p. 954-957 (2011); doi:10.1039/c0nr00822b
Descripción física:
p. 954-957
Abstract:

Herein, we demonstrate that both qualitative molecular and quantitative elemental data obtained from size exclusion chromatography coupled online for the first time to both molecular fluorescence and elemental mass spectrometry, respectively, turned out to be critical to evaluate the quality of coatings of quantum dots. Moreover, such an instrumental approach also allowed us to study quantitatively the appropriated bioconjugation of quantum dots to antibodies, a critical step for QDs future use in quantitative fluorescence immunoassays.

Herein, we demonstrate that both qualitative molecular and quantitative elemental data obtained from size exclusion chromatography coupled online for the first time to both molecular fluorescence and elemental mass spectrometry, respectively, turned out to be critical to evaluate the quality of coatings of quantum dots. Moreover, such an instrumental approach also allowed us to study quantitatively the appropriated bioconjugation of quantum dots to antibodies, a critical step for QDs future use in quantitative fluorescence immunoassays.

URI:
http://hdl.handle.net/10651/8412
ISSN:
2040-3364
Identificador local:

20110118

DOI:
10.1039/c0nr00822b
Patrocinado por:

The authors are grateful for financial support of the Spanish Ministry of Education and Science, MEC (CTQ2006-02309), and FEDER program co-financing funds.

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