Generation of New Derivatives of the Antitumor Antibiotic Mithramycin by Altering the Glycosylation Pattern through Combinatorial Biosynthesis
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This work was supported by grants from the Spanish Ministry of Education and Science to C.M. (BMC2002-03599 and BIO2005-04115), a grant of the US National Institutes of Health (CA 91901) to J.R., and from Red Temática de Investigación Cooperativa de Centros de Cáncer (Ministry of Health, Spain; ISCIII-RETIC RD06/0020/0026). M.P. was the recipient of a predoctoral fellowship of the Spanish Ministry of Science and Technology. We thank the University of Kentucky core laboratories for NMR spectroscopy and mass spectrometry for the usage of their instruments, and the mass spectrometry lab from the Department of Chemistry of U. Nebraska–Lincoln for the high-resolution FAB mass spectra. Pharmamar S.A. is acknowledged for helping us with the antitumor assays.
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