Mostrar el registro sencillo del ítem

Selenium speciation in rat colon tissues

dc.contributor.authorRoman, Marco
dc.contributor.authorFernández Sánchez, María Luisa 
dc.contributor.authorSanz Medel, Alfredo 
dc.contributor.authorGonzález Iglesias, Héctor 
dc.contributor.authorCescon, Paolo
dc.contributor.authorBarbante, Carlo
dc.date.accessioned2013-01-30T10:06:46Z
dc.date.available2013-01-30T10:06:46Z
dc.date.issued2011
dc.identifier.citationJournal of Analytical Atomic Spectrometry, 26(1), p. 100-108 (2011); doi:10.1039/c0ja00116cspa
dc.identifier.issn0267-9477
dc.identifier.urihttp://hdl.handle.net/10651/8197
dc.description.abstractA study of selenium (Se) speciation in rat colon tissues is presented. Four different procedures for the extraction of Se compounds were evaluated in terms of recovery and species preservation. Total Se in tissue and extracts was determined by ICP-MS and isotope dilution analysis. The selected and optimized protocol allowed an extraction of 43% of Se, while continuously bubbling nitrogen in the solution during the procedure was mandatory to prevent the oxidative degradation of selenoproteins. Speciation analysis was then performed on the extracts using size exclusion- and anion exchange-HPLC for species separation. A number of Se compounds were detected in rat colon extracts, and individually quantified by coupling HPLC-ICP-MS and species-unspecific on-line (post-column) isotope dilution analysis. Among the isolated selenospecies, the two major proteins glutathione peroxidase type 2 and thioredoxin reductase type 1 have been potentially identified by their molecular weight using MALDI-TOF-MS.spa
dc.format.extentp. 100-108spa
dc.language.isoeng
dc.relation.ispartofJournal of Analytical Atomic Spectrometryspa
dc.rights(c) Journal of Analytical Atomic Spectrometry
dc.titleSelenium speciation in rat colon tissuesspa
dc.typejournal article
dc.identifier.local20110082spa
dc.identifier.doi10.1039/c0ja00116c
dc.relation.publisherversionhttp://dx.doi.org/10.1039/c0ja00116cspa


Ficheros en el ítem

FicherosTamañoFormatoVer

No hay ficheros asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem