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Novel HPLC-ICP-MS strategy for the determination of β2-Transferrin, the biomarker of cerebrospinal fluid (CSF) leakage

dc.contributor.authorCastillo Busto, María Estela del 
dc.contributor.authorMontes Bayón, María 
dc.contributor.authorGarcía Alonso, José Ignacio 
dc.contributor.authorCaruso, Joseph A.
dc.contributor.authorSanz Medel, Alfredo
dc.identifier.citationAnalyst, 135(7), p. 1538-1540 (2010); doi:10.1039/c0an00207kspa
dc.description.abstractAsialo-Transferrin (S0) or β2-Transferrin is an accepted marker of cerebrospinal fluid (CSF) leakage from the subarachnoid space into the nasal or aural cavity as the result of a head trauma. At this time, the S0 detection method of choice is isoelectric focusing on polyacrylamide gel with direct immunofixation of transferrin (Tf) and silverstaining which are time-consuming techniques (> 5 h). The aim of this study is to present an alternative methodology for determination of Tf sialoforms present in CSF samples, specifically S0, based on the detection of the Fe associated with those forms. A double spiking methodology is developed by saturating the protein with isotopically enriched iron (57Fe) and conducting post-column Isotope Dilution Analysis (IDA) with 54Fe after separation of the different sialoforms by anion exchange chromatography. The results obtained have been validated using a certified serum (ERM®-DA470) and applied with satisfactory results to a pooled CSF sample.eng
dc.format.extentp. 1538-1540spa
dc.publisherRoyal Society of Chemistry
dc.subjectInductively Coupled Plasma/Mseng
dc.subjectQuantitative Organic Analyseseng
dc.subjectBiological Fluidseng
dc.titleNovel HPLC-ICP-MS strategy for the determination of β2-Transferrin, the biomarker of cerebrospinal fluid (CSF) leakageeng
dc.typejournal article

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