An accurate high-resolution melting method to genotype bovine β-casein

dc.contributor.author	Royo Martín, Luis José
dc.contributor.author	del Cerro, Ana
dc.contributor.author	Vicente, Fernando
dc.contributor.author	Carballal, Alfonso
dc.contributor.author	de la Roza, Begoña
dc.date.accessioned	2025-01-29T06:35:24Z
dc.date.available	2025-01-29T06:35:24Z
dc.date.issued	2013-11-11
dc.identifier.citation	European Food Research and Technology, 238(2), 295–298 (2014); doi:10.1007/s00217-013-2101-z
dc.identifier.uri	https://hdl.handle.net/10651/76361
dc.description.abstract	In the present study, we used high-resolution melting analysis to simultaneously identify the two mutations that taken together give us the genotype of the most common bovine β-casein variants. Allelic variants are detected in real time, and no post-PCR manipulations are required, therefore limiting costs and possible carryover contamination. Data can be copied to a Microsoft Excel spreadsheet for semiautomatic determination of the genotype. The method was validated using different DNA sources, in 122 samples from 12 different bovine populations, obtaining the first report on bovine β-casein genotypes for some of them.	spa
dc.format.extent	p. 295-298	spa
dc.language.iso	eng	spa
dc.relation.ispartof	European Food Research and Technology. Volumen 238	spa
dc.rights	© Springer-Verlag
dc.title	An accurate high-resolution melting method to genotype bovine β-casein	spa
dc.type	journal article	spa
dc.identifier.doi	10.1007/s00217-013-2101-z
dc.relation.publisherversion	https://doi.org/10.1007/s00217-013-2101-z

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