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Controlled silver electrodeposition on gold nanoparticle antibody tags for ultrasensitive prostate specific antigen sensing using electrochemical and optical smartphone detection

dc.contributor.authorRedondo Fernández, Guillermo 
dc.contributor.authorCid Barrio, Laura 
dc.contributor.authorFernández Fernández-Argüelles, María Teresa 
dc.contributor.authorEscosura Muñiz, Alfredo de la 
dc.contributor.authorSoldado Cabezuelo, Ana Belén 
dc.contributor.authorCosta Fernández, José Manuel 
dc.date.accessioned2024-10-09T06:19:02Z
dc.date.available2024-10-09T06:19:02Z
dc.date.issued2024
dc.identifier.citationTalanta, 275 (2024); doi:10.1016/j.talanta.2024.126095
dc.identifier.issn0039-9140
dc.identifier.urihttps://hdl.handle.net/10651/74967
dc.description.abstractOne of the current challenges in medicine is to achieve a rapid and unequivocal detection and quantification of extremely low levels of disease biomarkers in complex biological samples. Here, we present the development and analytical evaluation of a low-cost smartphone-based system designed for ultrasensitive detection of the prostatespecific antigen (PSA) using two detection alternatives: electrochemical or optical, by coupling the smartphone with a portable potentiostat or magnifying lenses. An antibody tagged with gold nanoparticles (AuNPs), and indium tin oxide coated polyethylene terephthalate platform (ITO-PET) have been used to develop a sandwichtype immunoassay. Then, a controlled silver electrodeposition on the AuNPs surface is carried out, enhancing their size greatly. Due to such strong nanoparticle-size amplification (from nm to μm), the final detection can be dual, by measuring current intensity or the number of silver-enlarged microstructures generated. The proposed strategies exhibited limit detections (LOD) of 102 and 37 fg/mL for electrochemical and optical detection respectively. The developed immunosensor reaches excellent selectivity and performance characteristics to quantify biomarkers at clinically relevant values without any pretreatment. These proposed procedures could be useful to check and verify possible recurrence after clinical treatment of tumors or even report levels of disease serum biomarkers in early stages.
dc.description.sponsorshipGuillermo Redondo-Fernández acknowledges his PhD grant (BP20-050) from the Asturias Regional Government (Spain). Alfredo de la Escosura-Muñiz acknowledges his “Ramón y Cajal” Research Fellow (RyC-2016-20299). This research was funded by Spanish Ministry of Science and Innovation (PID2020-117282RB-I00, MCI-20-PID2019- 109698GB-I00 and MCI-21-PID2020-115204RB-I00), and by Principado de Asturias GRUPIN IDI/2021/000081 co-financed by the Feder Programme of the European Union. Authors would like to thank to Marcos García Ocaña from Biotechnology Preparative Unit for his advice, Marta Alonso Guervós for the help handling the confocal microscope and Angel Martínez Nistal for the development of the custom macro, both from Photonics Microscopy and Image Processing Unit of University of Oviedo.
dc.language.isoeng
dc.relation.ispartofTalanta
dc.rights© 2024 The Authors
dc.rightsCC Reconocimiento – No Comercial – Sin Obra Derivada 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceScopus
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85190779283&doi=10.1016%2fj.talanta.2024.126095&partnerID=40&md5=4cb9f844def437eedfa0215a7d583f84
dc.titleControlled silver electrodeposition on gold nanoparticle antibody tags for ultrasensitive prostate specific antigen sensing using electrochemical and optical smartphone detection
dc.typejournal article
dc.identifier.doi10.1016/j.talanta.2024.126095
dc.relation.projectIDBP20-050
dc.relation.projectIDRyC-2016-20299
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-117282RB-I00/ES/SENSOR OPTOELECTRONICO PORTATIL Y ESTRATEGIAS DE MEDIDA PARA LA MONITORIZACION DESCENTRALIZADA EN APLICACIONES DE MEDIO AMBIENTE, AGROALIMENTACION Y CLINICAS BASADAS EN (BIO)N/ 
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109698GB-I00/ES/DESARROLLO DE NANOPLATAFORMAS ANALITICAS Y ESTRATEGIAS BASADAS EN ESPECTROMETRIA DE MASAS AVANZADAS PARA LA CUANTIFICACION DE (BIO)MOLECULAS EN MUESTRAS COMPLEJAS/ 
dc.relation.projectIDIDI/2021/000081
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.talanta.2024.126095
dc.rights.accessRightsopen access
dc.type.hasVersionVoR


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