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Phage lytic protein chapsh3b encapsulated in niosomes and gelatine films
dc.contributor.author | Marchianó, Verdiana | |
dc.contributor.author | Duarte, A. C. | |
dc.contributor.author | Agún, S. | |
dc.contributor.author | Luque Rodríguez, Susana | |
dc.contributor.author | Marcet Manrique, Ismael | |
dc.contributor.author | Fernández, Lucía | |
dc.contributor.author | Matos González, María | |
dc.contributor.author | Blanco López, María del Carmen | |
dc.contributor.author | García, P. | |
dc.contributor.author | Gutiérrez Cervelló, Gemma | |
dc.date.accessioned | 2024-07-11T07:34:30Z | |
dc.date.available | 2024-07-11T07:34:30Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | Microorganisms, 12(1), (2024); doi:10.3390/microorganisms12010119 | |
dc.identifier.issn | 2076-2607 | |
dc.identifier.uri | https://hdl.handle.net/10651/73718 | |
dc.description.abstract | Antimicrobial resistance (AMR) has emerged as a global health challenge, sparking worldwide interest in exploring the antimicrobial potential of natural compounds as an alternative to conventional antibiotics. In recent years, one area of focus has been the utilization of bacteriophages and their derivative proteins. Specifically, phage lytic proteins, or endolysins, are specialized enzymes that induce bacterial cell lysis and can be efficiently produced and purified following overexpression in bacteria. Nonetheless, a significant limitation of these proteins is their vulnerability to certain environmental conditions, which may impair their effectiveness. Encapsulating endolysins in vesicles could mitigate this issue by providing added protection to the proteins, enabling controlled release, and enhancing their stability, particularly at temperatures around 4 ◦C. In this work, the chimeric lytic protein CHAPSH3b was encapsulated within non-ionic surfactant-based vesicles (niosomes) created using the thin film hydrating method (TFH). These protein-loaded niosomes were then characterized, revealing sizes in the range of 30–80 nm, zeta potentials between 30 and 50 mV, and an encapsulation efficiency (EE) of 50–60%. Additionally, with the objective of exploring their potential application in the food industry, these endolysin-loaded niosomes were incorporated into gelatine films. This was carried out to evaluate their stability and antimicrobial efficacy against Staphylococcus aureus | |
dc.description.sponsorship | This work was funded by MCIN/AEI/10.13039/501100011033/FEDER, UE, grant number PID2022-140988OB-I00, awarded to P.G. and L.F. Moreover, this work was co-funded by the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant Agreement No. 813439 (Break Biofilms) and was also co-financed by Consejería de Educación y Ciencia del Principado de Asturias (AYUD/2021/52132). | |
dc.language.iso | eng | |
dc.relation.ispartof | Microorganisms | |
dc.rights | © 2024 by the authors. Licensee MDPI | |
dc.rights | CC Reconocimiento 4.0 Internacional | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Scopus | |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85183163848&doi=10.3390%2fmicroorganisms12010119&partnerID=40&md5=2582ebf5a5927cbe7a896aab9d0574e4 | |
dc.title | Phage lytic protein chapsh3b encapsulated in niosomes and gelatine films | |
dc.type | journal article | |
dc.identifier.doi | 10.3390/microorganisms12010119 | |
dc.relation.projectID | MCIN/AEI/10.13039/501100011033/FEDER | |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-140988OB-I00/ES/VALIDACION DE TECNICAS SOSTENIBLES PARA LA MEJORA DE LA CALIDAD Y SEGURIDAD DE QUESOS/ | |
dc.relation.projectID | AYUD/2021/52132 | |
dc.relation.publisherversion | http://dx.doi.org/10.3390/microorganisms12010119 | |
dc.rights.accessRights | open access |
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