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Phage lytic protein chapsh3b encapsulated in niosomes and gelatine films

dc.contributor.authorMarchianó, Verdiana 
dc.contributor.authorDuarte, A. C.
dc.contributor.authorAgún, S.
dc.contributor.authorLuque Rodríguez, Susana 
dc.contributor.authorMarcet Manrique, Ismael 
dc.contributor.authorFernández, Lucía
dc.contributor.authorMatos González, María 
dc.contributor.authorBlanco López, María del Carmen 
dc.contributor.authorGarcía, P.
dc.contributor.authorGutiérrez Cervelló, Gemma 
dc.date.accessioned2024-07-11T07:34:30Z
dc.date.available2024-07-11T07:34:30Z
dc.date.issued2024
dc.identifier.citationMicroorganisms, 12(1), (2024); doi:10.3390/microorganisms12010119
dc.identifier.issn2076-2607
dc.identifier.urihttps://hdl.handle.net/10651/73718
dc.description.abstractAntimicrobial resistance (AMR) has emerged as a global health challenge, sparking worldwide interest in exploring the antimicrobial potential of natural compounds as an alternative to conventional antibiotics. In recent years, one area of focus has been the utilization of bacteriophages and their derivative proteins. Specifically, phage lytic proteins, or endolysins, are specialized enzymes that induce bacterial cell lysis and can be efficiently produced and purified following overexpression in bacteria. Nonetheless, a significant limitation of these proteins is their vulnerability to certain environmental conditions, which may impair their effectiveness. Encapsulating endolysins in vesicles could mitigate this issue by providing added protection to the proteins, enabling controlled release, and enhancing their stability, particularly at temperatures around 4 ◦C. In this work, the chimeric lytic protein CHAPSH3b was encapsulated within non-ionic surfactant-based vesicles (niosomes) created using the thin film hydrating method (TFH). These protein-loaded niosomes were then characterized, revealing sizes in the range of 30–80 nm, zeta potentials between 30 and 50 mV, and an encapsulation efficiency (EE) of 50–60%. Additionally, with the objective of exploring their potential application in the food industry, these endolysin-loaded niosomes were incorporated into gelatine films. This was carried out to evaluate their stability and antimicrobial efficacy against Staphylococcus aureus
dc.description.sponsorshipThis work was funded by MCIN/AEI/10.13039/501100011033/FEDER, UE, grant number PID2022-140988OB-I00, awarded to P.G. and L.F. Moreover, this work was co-funded by the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant Agreement No. 813439 (Break Biofilms) and was also co-financed by Consejería de Educación y Ciencia del Principado de Asturias (AYUD/2021/52132).
dc.language.isoeng
dc.relation.ispartofMicroorganisms
dc.rights© 2024 by the authors. Licensee MDPI
dc.rightsCC Reconocimiento 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScopus
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85183163848&doi=10.3390%2fmicroorganisms12010119&partnerID=40&md5=2582ebf5a5927cbe7a896aab9d0574e4
dc.titlePhage lytic protein chapsh3b encapsulated in niosomes and gelatine films
dc.typejournal article
dc.identifier.doi10.3390/microorganisms12010119
dc.relation.projectIDMCIN/AEI/10.13039/501100011033/FEDER
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-140988OB-I00/ES/VALIDACION DE TECNICAS SOSTENIBLES PARA LA MEJORA DE LA CALIDAD Y SEGURIDAD DE QUESOS/ 
dc.relation.projectIDAYUD/2021/52132
dc.relation.publisherversionhttp://dx.doi.org/10.3390/microorganisms12010119
dc.rights.accessRightsopen access


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© 2024 by the authors. Licensee MDPI
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