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Adipose Derived Stem Cells for Corneal Wound Healing after Laser Induced Corneal Lesions in Mice

dc.contributor.authorZeppieri, Marco
dc.contributor.authorSalvetat, Maria Letizia
dc.contributor.authorBeltrami, Antonio
dc.contributor.authorCesselli, Daniela
dc.contributor.authorRusso, Rossella
dc.contributor.authorAlcalde, Ignacio
dc.contributor.authorMerayo Lloves, Jesús 
dc.contributor.authorBrusini, Paolo
dc.contributor.authorParodi, Pier Camillo
dc.identifier.citationJournal of Clinical Medicine, 6(12), p. 1-16 (2017); doi:10.3390/jcm6120115
dc.description.abstractThe aim of our study was to assess the clinical effectiveness of topical adipose derived stem cell (ADSC) treatment in laser induced corneal wounds in mice by comparing epithelial repair, inflammation, and histological analysis between treatment arms. Corneal lesions were performed on both eyes of 40 mice by laser induced photorefractive keratectomy. All eyes were treated with topical azythromycin bid for three days. Mice were divided in three treatment groups (n = 20), which included: control, stem cells and basic serum; which received topical treatment three times daily for five consecutive days. Biomicroscope ssessments and digital imaging were performed by two masked graders at 30, 54, 78, 100, and 172 h to analyze extent of fluorescein positive epithelial defect, corneal inflammation, etc. Immunohistochemical techniques were used in fixed eyes to assess corneal repair markers Ki67, α Smooth Muscle Actin (α-SMA) and E-Cadherin. The fluorescein positive corneal lesion areas were significantly smaller in the stem cells group on days 1 (p < 0.05),2 (p < 0.02) and 3. The stem cell treated group had slightly better and faster re epithelization than the serum treated group in the initial phases. Comparative histological data showed signs of earlier and better corneal repair in epithelium and stromal layers in stem cell treated eyes, which showed more epithelial layers and enhanced wound healing performance of Ki67, E-Cadherin, and α-SMA. Our study shows the potential clinical and histological advantages in the topical ADSC treatment for corneal lesions in micespa
dc.format.extentp. 1-16spa
dc.relation.ispartofJournal of Clinical Medicine, 6(12)spa
dc.rightsCC Reconocimiento 4.0 Internacional
dc.subjectAdipose derived stem cellsspa
dc.subjectCorneal wound healingspa
dc.subjectStem cell therapyspa
dc.titleAdipose Derived Stem Cells for Corneal Wound Healing after Laser Induced Corneal Lesions in Micespa

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