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Point-of-care detection of extracellular vesicles: Sensitivity optimization and multiple-target detection
dc.contributor.author | Oliveira Rodríguez, Myriam | |
dc.contributor.author | Serrano Pertierra, Esther | |
dc.contributor.author | Costa García, Agustín | |
dc.contributor.author | Martín, S. L. | |
dc.contributor.author | Mo, M. Y. | |
dc.contributor.author | Cernuda Morollón, Eva María | |
dc.contributor.author | Blanco López, María del Carmen | |
dc.date.accessioned | 2017-02-01T07:40:50Z | |
dc.date.available | 2017-02-01T07:40:50Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Biosensors and Bioelectronics, 87, p. 38-45 (2017); doi:10.1016/j.bios.2016.08.001 | |
dc.identifier.issn | 0956-5663 | |
dc.identifier.uri | http://hdl.handle.net/10651/39997 | |
dc.description.abstract | Extracellular vesicles (EVs) are membrane-bound nanovesicles delivered by different cellular lineages under physiological and pathological conditions. Although these vesicles have shown relevance as biomarkers for a number of diseases, their isolation and detection still has several technical drawbacks, mainly related with problems of sensitivity and time-consumed. Here, we reported a rapid and multiple-targeted lateral flow immunoassay (LFIA) system for the detection of EVs isolated from human plasma. A range of different labels (colloidal gold, carbon black and magnetic nanoparticles) was compared as detection probe in LFIA, being gold nanoparticles that showed better results. Using this platform, we demonstrated that improvements may be carried out by incorporating additional capture lines with different antibodies. The device exhibited a limit of detection (LOD) of 3.4×106 EVs/µL when anti-CD81 and anti-CD9 were selected as capture antibodies in a multiple-targeted format, and anti-CD63 labeled with gold nanoparticles was used as detection probe. This LFIA, coupled to EVs isolation kits, could become a rapid and useful tool for the point-of-care detection of EVs, with a total analysis time of two hours. | |
dc.description.sponsorship | The authors would like to thank Dr. Sánchez-Madrid for the gift of antibodies. Funding from projects CTQ2013-47396-R (Spanish Ministry of Economy and Competitivity), FC15-GRUPIN14-022(Regional Government of Asturias) is acknowledged. M. Oliveira Rodríguez thanks FICYT for her pre-doctoral grant. | |
dc.format.extent | p. 38-45 | |
dc.language.iso | eng | |
dc.relation.ispartof | Biosensors and Bioelectronics, 87 | |
dc.source | Scopus | |
dc.source.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84981287376&partnerID=40&md5=a073dffb7bbc57c6e216407736a80fe3 | |
dc.title | Point-of-care detection of extracellular vesicles: Sensitivity optimization and multiple-target detection | |
dc.type | journal article | |
dc.identifier.doi | 10.1016/j.bios.2016.08.001 | |
dc.relation.projectID | MINECO/CTQ2013-47396-R | |
dc.relation.projectID | FC15-GRUPIN14-022 | |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.bios.2016.08.001 |
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