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Control of Saccharomyces cerevisiae carboxypeptidase S (CPS1) gene expression under nutrient limitation

dc.contributor.authorBordallo Landa, Javier 
dc.contributor.authorSuárez Rendueles, María Paz
dc.identifier.citationYeast, 9(4), p. 339–349 (1993); doi:10.1002/yea.320090404
dc.description.abstractExpression of the vacuolar carboxypeptidase S (CPS1) gene in Saccharomyces cerevisiae is regulated by the availability of nutrients. Enzyme production is sensitive to nitrogen catabolite repression; i.e. the presence of ammonium ions maintains expression of the gene at a low level. Transfer of ammonium–glucose pre-grown cells to a medium deprived of nitrogen causes a drastic increase in CPS1 RNA level provided that a readily usable carbon source, such as glucose or fructose, is available to the cells. Derepression of the gene by nitrogen limitation is cycloheximide-insensitive. Neither glycerol, ethanol, acetate nor galactose support derepression of CPS1 expression under nitrogen starvation conditions. Non-metabolizable sugar analogs (2-deoxyglucose, 6-methyl-glucose or glucosamine) do not allow derepression of CPS1, showing that the process is energy-dependent. Production of carboxypeptidase yscS also increases several-fold when ammonium-pregrown cells are transferred to media containing glucose and a non-readily metabolizable nitrogen source such as proline, leucine, valine or leucyl-glycine. Analysis of CPS1 expression in RAS2+ (high cAMP) and ras2 mutant (low cAMP) strains and in cells grown at low temperature (23°C) and in heat-shocked cells (38°C) shows that steady-state levels of CPS1 mRNA are not controlled by a low cAMP level-signalling pathwayen
dc.format.extentp. 339-349spa
dc.relation.ispartofYeast, 9(4)spa
dc.titleControl of Saccharomyces cerevisiae carboxypeptidase S (CPS1) gene expression under nutrient limitationen

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