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Metal-metallothioneins like proteins investigation by heteroatom-tagged proteomics in two different snails as possible sentinel organisms of metal contamination in freshwater ecosystems

dc.contributor.authorFrança Maltez, Heloísa
dc.contributor.authorVillanueva Tagle, Margarita
dc.contributor.authorFernández de la Campa, María del Rosario 
dc.contributor.authorSanz Medel, Alfredo 
dc.date.accessioned2013-01-30T10:17:57Z
dc.date.available2013-01-30T10:17:57Z
dc.date.issued2009
dc.identifier.citationAnalytica Chimica Acta, 650(2), p. 234-240 (2009); doi:10.1016/j.aca.2009.07.052spa
dc.identifier.issn0003-2670
dc.identifier.urihttp://hdl.handle.net/10651/10265
dc.description.abstractMetal speciation analysis in MLPs was carried out in two snails, Marisa cornuarietis and Pomacea bridgesi, in order to investigate them as possible sentinel organisms of heavy metal contamination. To carry out this study snails born in a non-contaminated environment were divided into two groups: a control group and a contaminated one with cadmium administered for 40 days. Subsequently, we investigated the speciation of the induced MLPs in exposed animals in relation to controls. In order to obtain the MLP fraction, cytosols from both snail species where subjected to size-exclusion fractionation, monitoring on-line the metal signal (Cd, Cu and Zn) by ICP-MS while protein elution was followed by on-line UV detection. MLP fraction was then separated by anion-exchange (AE)-FPLC using optimal chromatographic conditions for the separation of the different MLP isoforms in both snail species. Specific detection of separated metalloforms was carried out again by the hyphenation of the AE chromatographic system with ICP-MS. The determination of the amount of metal bound to MLPs was carried out by post-column isotope dilution analysis ICP-MS, finding that the snail M. cornuarietis accumulated higher concentrations of cadmium than P. bridgesi. Thus this first snail could therefore be a better candidate sentinel organism of pollution in natural waters. Identification and characterization of the isoforms separated in M. cornuarietis was carried out for the entire or intact isoforms by MALDI-TOF and then conventional triptic digestion was also carried out to identify the nature of the formed peptides. The presence identification of a MLP isoform of relatively low molecular weight in M. cornuarietis is reported.spa
dc.format.extentp. 234-240spa
dc.language.isoeng
dc.relation.ispartofAnalytica Chimica Actaspa
dc.rights(c) Analytica Chimica Acta
dc.sourceSCOPUSspa
dc.source.urihttp://www.scopus.com/inward/record.url?eid=2-s2.0-69249102935&partnerID=40
dc.subjectMetallothionein Like Proteins; Metal Speciation; Inductively Coupled Plasma Mass Spectrometry; Marisa Cornuarietis and Pomacea Bridgesi; Sentinel Organismsspa
dc.titleMetal-metallothioneins like proteins investigation by heteroatom-tagged proteomics in two different snails as possible sentinel organisms of metal contamination in freshwater ecosystemsspa
dc.typejournal article
dc.identifier.local20090294spa
dc.identifier.doi10.1016/j.aca.2009.07.052
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.aca.2009.07.052spa


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