A New Scaffold of an Old Protein Fold Ensures Binding to the Bisintercalator Thiocoraline
This work was supported by start-up funds from the Life Sciences Institute (to S.G.-T.) and the College of Pharmacy (to S.G.-T. and O.V.T.) at the University of Michigan and by the Vahlteich Research Award Fund, College of Pharmacy at the University of Michigan (to S.G.-T.). Research performed at JAS laboratory was funded by Red Temática de Investigación Cooperativa de Centros de Cáncer (ISCIII-RETIC RD06/0020/0026; Ministry of Health, Spain). We thank Jessica M. Pero for the cloning of the Histagged wild-type TioX and for the preparation of its SeMet-substituted variant. We thank Dr. Spencer Anderson and the staff of sectors LS-CAT and GM/CA-CAT at the Advanced Photon Source at the Argonne National Laboratory for assistance with the X-ray diffraction data collection.
- Artículos