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Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water

dc.contributor.authorGutiérrez del Río Menéndez, Ignacio 
dc.contributor.authorMarín Fernández, Laura 
dc.contributor.authorFernández Fernández, Javier 
dc.contributor.authorÁlvarez San Millán, María 
dc.contributor.authorFerrero Martín, Francisco Javier 
dc.contributor.authorValledor Llopis, Marta 
dc.contributor.authorCampo Rodríguez, Juan Carlos 
dc.contributor.authorCobián Fernández, Natalia
dc.contributor.authorMéndez Balbuena, Ignacio
dc.contributor.authorLombó Brugos, Felipe 
dc.date.accessioned2018-04-24T09:39:29Z
dc.date.available2018-04-24T09:39:29Z
dc.date.issued2018
dc.identifier.citationPLoS ONE, 13(1), p. e0184277- (2018); doi:10.1371/journal.pone.0184277
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10651/46667
dc.description.sponsorshipThis work received support from FICYT IE09-106 and IDEPA IDE/2012/000452. NC and IM belong to HIPSITEC SA and were taking part in this study in the following activities: NC in the conceptualization, investigation (construction of some of the plasmids for expression of the magic bullet biosensor proteins in S.albus), methodology (designing in part of the analyzer device for optical excitation and signal detection of the bound proteins to bacteria), supervision (of the engineering part of the project) and funding acquisition (proposals coordination and submission). IM in the methodology (tests with the device for checking signal detection parameters) and software (for controlling the LED-PMT-INTERFACE parts). We thank financial support from FICYT (Fundacion para el Fomento en Asturias de la Investigacion Cientifica Aplicada y la Tecnologia) and IDEPA (Instituto de Desarrollo Economico del Principado de Asturias) through the Projects IE09-106 and IDE2012/000452 to HIPSITEC SA. We also thank Servicios Cientifico-Tecnicos from the University of Oviedo for technical support in protein purification.
dc.format.extentp. e0184277-
dc.language.isoeng
dc.relation.ispartofPLoS ONE, 13
dc.rights© 2018 Gutiérrez-del-Río et al.
dc.rightsCC Reconocimiento 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScopus
dc.source.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85040193604&doi=10.1371%2fjournal.pone.0184277&partnerID=40&md5=933e216e34dd338638e187df0ca759ed
dc.titleDevelopment of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
dc.typejournal article
dc.identifier.doi10.1371/journal.pone.0184277
dc.relation.projectIDFICYT/IE09-106
dc.relation.projectIDIDEPA/IDE2012/000452
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0184277
dc.rights.accessRightsopen access


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© 2018 Gutiérrez-del-Río et al.
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