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We thank S. Glise (INRA, France) for taking care of the fish at the Lapitxuri experimental station, and I. Gonzalez Pola (University of Oviedo, Spain) for technical assistance. A. Salas (University of Oviedo) performed cytometry analyses. Prof F. Juanes (University of Massachusetts, USA) kindly revised the manuscript. The authors are indebted to Prof J. C. Avise and Prof M. Arnold (University of Georgia, USA), to Dr L. Bernatchez (University of Laval, Canada), to Prof R. Giraldez (University of Oviedo, Spain) and to Dr P. Gaudin (INRA, France) for precious comments on this article. E.G.V., recipient of a grant from the Spanish government for a short stay at the University of Georgia (Ref. PR2004-0084), is personally grateful to J. L. Chapuis, S. Chilmonczyk and D. Delille (IPEV, France) for their support on hybridization matters. This study was funded by the Spanish National Grant MCYT REN2003-0303.