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High frequency lateral flow affinity assay using superparamagnetic nanoparticles

dc.contributor.authorLago Cachón, David 
dc.contributor.authorRivas Ardisana, Montserrat 
dc.contributor.authorMartínez García, José Carlos 
dc.contributor.authorOliveira Rodríguez, Myriam 
dc.contributor.authorBlanco López, María del Carmen 
dc.contributor.authorGarcía Díaz, José Ángel 
dc.date.accessioned2017-02-09T12:24:56Z
dc.date.available2017-02-09T12:24:56Z
dc.date.issued2017
dc.identifier.citationJournal of Magnetism and Magnetic Materials, 423, p. 436-440 (2017); doi:10.1016/j.jmmm.2016.09.106
dc.identifier.issn0304-8853
dc.identifier.urihttp://hdl.handle.net/10651/40119
dc.description.abstractLateral flow assay is one of the simplest and most extended techniques in medical diagnosis for point-of-care testing. Although it has been traditionally a positive/negative test, some work has been lately done to add quantitative abilities to lateral flow assay. One of the most successful strategies involves magnetic beads and magnetic sensors. Recently, a new technique of superparamagnetic nanoparticle detection has been reported, based on the increase of the impedance induced by the nanoparticles on a RF-current carrying copper conductor. This method requires no external magnetic field, which reduces the system complexity. In this work, nitrocellulose membranes have been installed on the sensor, and impedance measurements have been carried out during the sample diffusion by capillarity along the membrane. The impedance of the sensor changes because of the presence of magnetic nanoparticles. The results prove the potentiality of the method for point-ofcare testing of biochemical substances and nanoparticle capillarity flow studies.spa
dc.description.sponsorshipThis work was partly supported by the Spanish Government Projects MINECO-13-MAT2012-33405 and MINECO-13-CTQ2013-47396-R, and Principality of Asturias Project GRUPIN14-037. M. Oliveira-Rodríguez thanks FICYT (Asturias, Spain) for grant BP14-005, and D. Lago-Cachón thanks Instituto Universitario de Tecnología Industrial de Asturias and the Council of Gijón for grant SV-14-GIJÓN- 1.10.
dc.format.extentp. 436-440spa
dc.language.isoengspa
dc.relation.ispartofJournal of Magnetism and Magnetic Materials, 423spa
dc.titleHigh frequency lateral flow affinity assay using superparamagnetic nanoparticlesspa
dc.typejournal articlespa
dc.relation.projectIDMINECO-13-MAT2012-33405
dc.relation.projectIDMINECO-13-CTQ2013-47396-R
dc.relation.projectIDGRUPIN14-037
dc.relation.projectIDSV-14-GIJÓN-1.10
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.jmmm.2016.09.106spa


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