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Affinity of aptamers binding 33-mer gliadin peptide and gluten proteins: Influence of immobilization and labeling tags

dc.contributor.authorAmaya González, Sonia 
dc.contributor.authorLópez López, Laura
dc.contributor.authorMiranda Castro, Rebeca 
dc.contributor.authorSantos Álvarez, Noemí de los 
dc.contributor.authorMiranda Ordieres, Arturo José 
dc.contributor.authorLobo Castañón, María Jesús 
dc.date.accessioned2015-08-20T10:07:46Z
dc.date.available2015-08-20T10:07:46Z
dc.date.issued2015
dc.identifier.citationAnalytica Chimica Acta, 873, p. 63-70 (2015); doi:10.1016/j.aca.2015.02.053
dc.identifier.issn0003-2670
dc.identifier.issn1873-4324
dc.identifier.urihttp://hdl.handle.net/10651/32546
dc.description.abstractAptamers are starting to increase the reagents tool box to develop more sensitive and reliable methods for food allergens. In most of these assays, aptamers have to be modified for detection and/or immobilization purposes. To take full advantage of their affinity, which decisively influence the detectability, these modifications must be faced rationally. In this work, a recently developed aptamer for an immunotoxic peptide of gliadin associated to celiac disease is used in different configurations and modified with various markers and anchored groups to evaluate the influence of such modifications on the real affinity...spa
dc.description.sponsorshipThis work was financed by Spanish Government Project CTQ2012-31157, and the European Regional Development Fund.
dc.format.extentp. 63-70spa
dc.language.isoengspa
dc.publisherElsevier
dc.relation.ispartofAnalytica Chimica Acta 873spa
dc.subjectAptamerspa
dc.subjectGlutenspa
dc.subjectBinding constantspa
dc.titleAffinity of aptamers binding 33-mer gliadin peptide and gluten proteins: Influence of immobilization and labeling tagsspa
dc.typejournal article
dc.identifier.doi10.1016/j.aca.2015.02.053
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.aca.2015.02.053


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