Dipeptidyl aminopeptidase yspI mutants of Schizosaccharomyces pombe: genetic mapping of dpa1+ on chromosome III

dc.contributor.author	Villa, Lourdes
dc.contributor.author	Suárez Rendueles, María Paz
dc.date.accessioned	2014-11-28T10:56:41Z
dc.date.available	2014-11-28T10:56:41Z
dc.date.issued	1994
dc.identifier.citation	FEMS Microbiology Letters, 120(1), p. 211–216 (1994)
dc.identifier.issn	0378-1097
dc.identifier.issn	1574-6968
dc.identifier.uri	http://hdl.handle.net/10651/28894
dc.description.abstract	A mutant strain of Schizosaccharomyces pombe lacking dipeptidyl aminopeptidase yspI was isolated from a strain already defective in aminopeptidase activity by means of a staining technique with the chromogenic substrate ala-pro-4-methoxy-beta-naphthylamide to screen colonies for the absence of the enzyme. The defect segregated 2(+):2(-) in meiotic tetrads, indicating a single chromosomal gene mutation, which was shown to be recessive. Gene dosage experiments indicated that the mutation resides in the structural gene of dipeptidyl aminopeptidase yspI, dpa1(+). The dpa1(+) gene was located on chromosome III by using m-fluorophenylalanine-induced haploidization and mitotic analysis, dpa1 mutants did not show any obvious phenotype under a variety of conditions tested	spa
dc.format.extent	p. 211-216	spa
dc.language.iso	eng	spa
dc.publisher	Wiley
dc.relation.ispartof	FEMS Microbiology Letters, 120(1)	spa
dc.title	Dipeptidyl aminopeptidase yspI mutants of Schizosaccharomyces pombe: genetic mapping of dpa1+ on chromosome III	en
dc.type	journal article

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