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Enriched stable isotopes and isotope pattern deconvolution for quantitative speciation of endogenous and exogenous selenium in rat urine by HPLC-ICP-MS

dc.contributor.authorGonzález Iglesias, Héctor 
dc.contributor.authorFernández Sánchez, María Luisa 
dc.contributor.authorRodríguez Castrillón, José Ángel 
dc.contributor.authorGarcía Alonso, José Ignacio 
dc.contributor.authorLópez Sastre, José Blas 
dc.contributor.authorSanz Medel, Alfredo 
dc.date.accessioned2013-01-30T10:19:02Z
dc.date.available2013-01-30T10:19:02Z
dc.date.issued2009
dc.identifier.citationJournal of Analytical Atomic Spectrometry, 24, p. 460-468 (2009); doi:10.1039/b820297bspa
dc.identifier.issn0267-9477
dc.identifier.urihttp://hdl.handle.net/10651/10469
dc.description.abstractInterest in selenium metabolism and its role in health and disease continue to stimulate a lot of bioanalytical research. The use of enriched stable isotopes of Se and isotope pattern deconvolution (IPD) with an ICP-MS in lactating rats is approached here for total selenium distribution studies. A mathematical tool has been developed to calculate supplemented and endogenous total Se contents in urine and faeces by ICP-MS. Also, quantification of endogenous (natural) and exogenous seleno-species by HPLC-ICP-MS has been worked out. The proposed IPD methodology, for total determinations and for quantitative speciation, was applied to reference materials to validate total selenium quantification in faeces and quantitative selenium speciation in urine samples. Selenium apparent absorption and retention and natural and exogenous selenium distribution in urine samples were calculated. Interesting quantitative information about selenium bio-transformations and final catabolism can be obtained from careful examination of the results observed, using such analytical tools, of the urinary endogenous and exogenous selenium metabolites in urine of rats and their kinetics throughout the two weeks of selenium supplementation.eng
dc.format.extentp. 460-468spa
dc.language.isoeng
dc.relation.ispartofJournal of Analytical Atomic Spectrometryeng
dc.rights© The Royal Society of Chemistry 2009
dc.titleEnriched stable isotopes and isotope pattern deconvolution for quantitative speciation of endogenous and exogenous selenium in rat urine by HPLC-ICP-MSeng
dc.typejournal article
dc.identifier.local20090699spa
dc.identifier.doi10.1039/b820297b
dc.relation.publisherversionhttp://dx.doi.org/10.1039/b820297bspa


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