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Evaluation of antiproliferative activities and apoptosis induction caused by copper(II)-benzothiazolesulfonamide complexes in Jurkat T lymphocytes and Caco-2 cells

dc.contributor.authorGonzález Álvarez, Marta
dc.contributor.authorAlzuet Piña, Gloria
dc.contributor.authorBorrás Tortonda, Joaquín
dc.contributor.authorCastillo Agudo, Lucas del
dc.contributor.authorMontejo Bernardo, José Manuel 
dc.contributor.authorGutiérrez Rodríguez, Ángel 
dc.contributor.authorGarcía-Granda, Santiago 
dc.date.accessioned2013-01-30T10:18:44Z
dc.date.available2013-01-30T10:18:44Z
dc.date.issued2008
dc.identifier.citationJournal of Biological Inorganic Chemistry, 13(8), p. 1249-1265 (2008); doi:10.1007/s00775-008-0409-0spa
dc.identifier.issn0949-8257
dc.identifier.urihttp://hdl.handle.net/10651/10417
dc.description.abstractTwo copper(II) complexes with a benzothiazolesulfonamide ligand, [Cu(L)2(py)2] (1) and [Cu(en)2(L)2] (2) [HL is N-2-(4-methylbenzothiazole)toluenesulfonamide, py is pyridine, en is ethylenediamine], were prepared and then characterized with the aid of X-ray crystallography and spectroscopy. Whereas the copper(II) ion in 1 presents a square-planar geometry, in 2 it has a distorted octahedral environment. In addition, although the ligand is monodentate in both complexes, it exhibits different coordination behavior in each, interacting through the benzothiazole nitrogen atom in 1 and through the sulfonamide nitrogen atom in 2. The propensity for binding of 1 and 2 to calf thymus DNA was studied by thermal denaturation, viscosimetry, and cyclic voltammetry. The ability of the complexes to cleave DNA was studied in vitro through ascorbate activation and was tested by monitoring the expression of the yEGFP gene containing the RAD54 reporter. Moreover, their antiproliferative activity was verified in two cellular models: yeast and human tumor cells in culture. While 1 was found to be the more active cleaving agent in vitro, 2 showed a higher propensity for inflicting DNA damage at the cellular level. The biological studies carried out with human tumor cells, namely, colon adenocarcinoma Caco-2 cells (HTB-37) and leukemia Jurkat Tlymphocytes (TIB-152), confirmed that both compounds inhibit the growth of these cell lines, although 2 is more effective. This difference is associated with the latter compound’s greater ability to induce cell death by apoptosis.spa
dc.format.extentp. 1249-1265spa
dc.language.isoeng
dc.relation.ispartofJournal of Biological Inorganic Chemistryspa
dc.rights(c) Journal of Biological Inorganic Chemistry
dc.sourceWOKspa
dc.subjectCopper(Ii)–Sulfonamide Complexes. Genotoxicity. Cell Proliferation Inhibition. Apoptosisspa
dc.titleEvaluation of antiproliferative activities and apoptosis induction caused by copper(II)-benzothiazolesulfonamide complexes in Jurkat T lymphocytes and Caco-2 cellsspa
dc.typeinfo:eu-repo/semantics/article
dc.identifier.local2538spa
dc.identifier.doi10.1007/s00775-008-0409-0
dc.type.dcmitextspa
dc.relation.publisherversionhttp://dx.doi.org/10.1007/s00775-008-0409-0spa


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