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Please use this identifier to cite or link to this item: http://hdl.handle.net/10651/8472

Title: Quantum dot-based array for sensitive detection of Escherichia coli
Author(s): Sanvicens Díez, Nuria
Pascual Durán, Nuria
Fernández Fernández-Argüelles, María Teresa
Adrián Izquierdo, Javier
Costa Fernández, José Manuel
Sánchez Baeza, Francisco
Sanz Medel, Alfredo
Marco Colás, María Pilar
Keywords: Bacteria . E. Coli . Antibody Array . Quantum Dot
Issue date: 2011
Publisher version: http://dx.doi.org/10.1007/s00216-010-4624-5
Citation: Analytical and Bioanalytical Chemistry, 399(8), p. 1-8 (2011); doi:10.1007/s00216-010-4624-5
Format extent: p. 1-8
Abstract: A fluorescent quantum dot-based antibody array, used in sandwich format, has been developed to detect Escherichia coli O157:H7. Numerous parameters such as solid support, optimal concentration of immunoreagents, blocking reagents, and assay time were optimized for array construction. Quantum dot-conjugated anti-IgG was used as the detecting system. The array allows the detection of E. coli O157:H7 at concentrations below 10 CFU mL−1 without sample enrichment, exhibiting an increase of three orders of magnitude in the limit of detection compared to ELISA. The interference caused by Gram (+) and Gram (−) bacteria was negligible at low concentrations of bacteria.
URI: http://hdl.handle.net/10651/8472
ISSN: 1618-2642
Local identifier: 20110078
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