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Repositorio de la Universidad de Oviedo. > Producción Bibliográfica de UniOvi: RECOPILA > Artículos >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10651/7651

Title: Novel HPLC-ICP-MS strategy for the determination of β2-Transferrin, the biomarker of cerebrospinal fluid (CSF) leakage
Author(s): Castillo Busto, María Estela del
Montes Bayón, María
García Alonso, José Ignacio
Caruso, Joseph A.
Sanz Medel, Alfredo
Keywords: Inductively Coupled Plasma/Ms
Lc/Ms
Quantitative Organic Analyses
Biomarkers
Transferrin
Biological Fluids
Issue date: 2010
Publisher: Royal Society of Chemistry
Publisher version: http://dx.doi.org/10.1039/c0an00207k
Citation: Analyst, 135(7), p. 1538-1540 (2010); doi:10.1039/c0an00207k
Format extent: p. 1538-1540
Abstract: Asialo-Transferrin (S0) or β2-Transferrin is an accepted marker of cerebrospinal fluid (CSF) leakage from the subarachnoid space into the nasal or aural cavity as the result of a head trauma. At this time, the S0 detection method of choice is isoelectric focusing on polyacrylamide gel with direct immunofixation of transferrin (Tf) and silverstaining which are time-consuming techniques (> 5 h). The aim of this study is to present an alternative methodology for determination of Tf sialoforms present in CSF samples, specifically S0, based on the detection of the Fe associated with those forms. A double spiking methodology is developed by saturating the protein with isotopically enriched iron (57Fe) and conducting post-column Isotope Dilution Analysis (IDA) with 54Fe after separation of the different sialoforms by anion exchange chromatography. The results obtained have been validated using a certified serum (ERM®-DA470) and applied with satisfactory results to a pooled CSF sample.
URI: http://hdl.handle.net/10651/7651
ISSN: 0003-2654
Local identifier: 20100506
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