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Repositorio de la Universidad de Oviedo. > Producción Bibliográfica de UniOvi: RECOPILA > Artículos >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10651/7528

Title: Enantioselective determination of thyroxine enantiomers by ligand-exchange CE with UV absorbance and ICP-MS detection
Author(s): Kang, Jianzhen
Kutscher, Daniel Jurgen
Montes Bayón, María
Blanco González, Elisa
Sanz Medel, Alfredo
Keywords: Ce-Icp-Ms; Chiral Speciation; Enantioselective Ce; Ligand-Exchange; Thyroxine Enantiomers
Issue date: 2009
Publisher version: http://dx.doi.org/10.1002/elps.200800731
Citation: Electrophoresis, 30(10), p. 1774-1782 (2009); doi:10.1002/elps.200800731
Format extent: p. 1774-1782
Abstract: A simple CE method has been developed for the separation and determination of thyroxine (T4) enantiomers in pharmaceutical formulations. The method was based on ligand-exchange mechanism using a Cu(II)/L-proline complex as chiral selector. The effects of different parameters affecting separation such as chiral selector concentration, organic additive, buffer pH and temperature were investigated. A baseline separation of the two enantiomers was obtained at a Cu(II)/L-proline ratio of 1:8 in a borate buffer (15 mmol/L, pH 9.6) containing 10% v/v acetonitrile. Under the optimized conditions, precision linearity range and detection limits of the developed enantioselective CE method were evaluated and compared using two different detection systems: conventional UV detection at 226 nm and iodine (127I)specific detection (“chiral speciation”) with ICP-MS. Both methodologies show adequate analytical performance characteristics with detection limits around 0.30 μg/mL for each enantiomer of T4. Finally, a levothroid pharmaceutical formulation sample was successfully analyzed using both developed methods CE-UV and CE-ICP-MS.
URI: http://hdl.handle.net/10651/7528
ISSN: 0173-0835
Local identifier: 20090604
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