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Please use this identifier to cite or link to this item: http://hdl.handle.net/10651/46667

Title: Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
Author(s): Gutiérrez del Río Menéndez, Ignacio
Marín Fernández, Laura
Fernández Fernández, Javier
Álvarez San Millán, María
Ferrero Martín, Francisco Javier
Valledor Llopis, Marta
Campo Rodríguez, Juan Carlos
Cobián Fernández, Natalia
Méndez Balbuena, Ignacio
Lombó Brugos, Felipe
Issue date: 2018
Publisher version: http://dx.doi.org/10.1371/journal.pone.0184277
Citation: PLoS ONE, 13(1), p. e0184277- (2018); doi:10.1371/journal.pone.0184277
Format extent: p. e0184277-
URI: http://hdl.handle.net/10651/46667
ISSN: 1932-6203
Sponsored: This work received support from FICYT IE09-106 and IDEPA IDE/2012/000452. NC and IM belong to HIPSITEC SA and were taking part in this study in the following activities: NC in the conceptualization, investigation (construction of some of the plasmids for expression of the magic bullet biosensor proteins in S.albus), methodology (designing in part of the analyzer device for optical excitation and signal detection of the bound proteins to bacteria), supervision (of the engineering part of the project) and funding acquisition (proposals coordination and submission). IM in the methodology (tests with the device for checking signal detection parameters) and software (for controlling the LED-PMT-INTERFACE parts). We thank financial support from FICYT (Fundacion para el Fomento en Asturias de la Investigacion Cientifica Aplicada y la Tecnologia) and IDEPA (Instituto de Desarrollo Economico del Principado de Asturias) through the Projects IE09-106 and IDE2012/000452 to HIPSITEC SA. We also thank Servicios Cientifico-Tecnicos from the University of Oviedo for technical support in protein purification.
Project id.: FICYT/IE09-106
Appears in Collections:Biología Funcional
Investigaciones y Documentos OpenAIRE

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