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Use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10651/44479

Título : Bioelectroanalysis in a Drop: Construction of a Glucose Biosensor
Autor(es) y otros: Amor Gutiérrez, Olaya
Costa Rama, Estefanía
Fernández Abedul, María Teresa
Costa García, Agustín
Palabras clave: Upper-Division Undergraduate
Analytical Chemistry
Fecha de publicación : 2017
Versión del editor: http://dx.doi.org/10.1021/acs.jchemed.6b00948
Citación : Journal of Chemical Education, 94(6), p. 806−812 (2017); doi:10.1021/acs.jchemed.6b00948
Descripción física: p. 806–812
Resumen : This lab experiment describes a complete method to fabricate an enzymatic glucose electroanalytical biosensor by students. Using miniaturized and disposable screen-printed electrodes (SPEs), students learn how to use them as transducers and understand the importance SPEs have acquired in sensor development during the last years. Students can also revise concepts related to enzymatic assays, with glucose oxidase and horseradish peroxidase involved in subsequent reactions. Moreover, they learn the trends that current analytical chemistry follows presently such as miniaturization, portability, and low cost. At the same time, this experiment serves to teach basic analytical concepts (accuracy, precision, sensitivity, and selectivity) in a practical way. The high clinical interest of glucose, due to a large number of diabetes patients around the world, and the application of the sensor to analysis of real food samples make this experiment very attractive to students. The questions set out along this experiment help students to acquire skills for solving analytical problems from the very beginning.
URI : http://hdl.handle.net/10651/44479
ISSN : 0021-9584
Aparece en las colecciones: Química Física y Analítica
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