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Please use this identifier to cite or link to this item: http://hdl.handle.net/10651/44479

Title: Bioelectroanalysis in a Drop: Construction of a Glucose Biosensor
Author(s): Amor Gutiérrez, Olaya
Costa Rama, Estefanía
Fernández Abedul, María Teresa
Costa García, Agustín
Keywords: Upper-Division Undergraduate
Analytical Chemistry
Issue date: 2017
Publisher version: http://dx.doi.org/10.1021/acs.jchemed.6b00948
Citation: Journal of Chemical Education, 94(6), p. 806−812 (2017); doi:10.1021/acs.jchemed.6b00948
Format extent: p. 806–812
Abstract: This lab experiment describes a complete method to fabricate an enzymatic glucose electroanalytical biosensor by students. Using miniaturized and disposable screen-printed electrodes (SPEs), students learn how to use them as transducers and understand the importance SPEs have acquired in sensor development during the last years. Students can also revise concepts related to enzymatic assays, with glucose oxidase and horseradish peroxidase involved in subsequent reactions. Moreover, they learn the trends that current analytical chemistry follows presently such as miniaturization, portability, and low cost. At the same time, this experiment serves to teach basic analytical concepts (accuracy, precision, sensitivity, and selectivity) in a practical way. The high clinical interest of glucose, due to a large number of diabetes patients around the world, and the application of the sensor to analysis of real food samples make this experiment very attractive to students. The questions set out along this experiment help students to acquire skills for solving analytical problems from the very beginning.
URI: http://hdl.handle.net/10651/44479
ISSN: 0021-9584
Sponsored: This work was supported by the FC-15-GRUPIN-021 Project from the Asturias Regional Government and the CTQ2014- 58826-R Project from the Spanish Ministry of Economy and Competitiveness (MINECO).
Project id.: Asturias Regional Government/FC-15-GRUPIN-021
MINECO/CTQ2014- 58826-R
Appears in Collections:Química Física y Analítica
Investigaciones y Documentos OpenAIRE

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