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Please use this identifier to cite or link to this item: http://hdl.handle.net/10651/37263

Title: A novel handheld fluorimeter for rapid detection of escherichia coli in drinking water
Author(s): Ferrero Martín, Francisco Javier
Valledor Llopis, Marta
Campo Rodríguez, Juan Carlos
Marín Fernández, Laura
Gutiérrez del Río Menéndez, Ignacio
Fernández Fernández, Javier
Lombó Brugos, Felipe
Cobián Fernández, Natalia
Olmos Fernández-Corugedo, Francisco
Méndez Suárez, Irene
Keywords: Drinking water
Escherichia coli
Green fluorescent protein
Issue date: 2016
Publisher: IEEE
Publisher version: http://dx.doi.org/10.1109/JSEN.2016.2558649
Citation: IEEE Sensors Journal, 16(13), p. 5136-5144 (2016); doi:10.1109/JSEN.2016.2558649
Abstract: The microbiological quality of drinking water is a concern to consumers, water suppliers, regulators and public health authorities alike. Monitoring the microbiological quality of drinking water relies largely on examination of indicator bacteria such as coliforms like Escherichia coli. E. coli is widely used as an indicator of fecal pollution when monitoring the microbial quality of drinking water because it is abundant in all mammal feces and therefore is found in sewage and in natural waters contaminated with fecal matter, from human origin, wild animals or derived from agricultural activity. This paper describes the development of a novel handheld fluorimeter for rapid detection of E. coli in drinking water based on a specific cellular biomarker. The measurement system is based on a photomultiplier tube that captures the fluorescence signal produced by the cellular biomarker when it is excited by an ultraviolet LED. The cellular biomarker is also developed and it consists of a chimeric protein with a Green fluorescent protein in the N-terminal domain (GFP) and a specific amino acid sequence in the C-terminal domain (Colicin S4) that targets specifically the structure of the microorganism to be detected. The instrument is simple to use, lightweight, and can be powered by either an AC/DC power adapter or a rechargeable battery, making it an excellent choice for rapid detection of E. coli in drinking water in field studies and laboratory measurements
URI: http://hdl.handle.net/10651/37263
ISSN: 1530-437X
Sponsored: Financial support from IDEPA (Instituto de Desarrollo Económico del Principado de Asturias) and FICYT (Fundación para el Fomento en Asturias de la Investigación Científica Aplicada y la Tecnología) through the projects IE09-106 and IDE2012/000452 is gratefully acknowledged
Project id.: Principado de Asturias/IE09-106
Principado de Asturias/IDE2012-000452
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Ingeniería Eléctrica, Electrónica, de Computadores y Sistemas
Investigaciones y Documentos OpenAIRE

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