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Please use this identifier to cite or link to this item: http://hdl.handle.net/10651/32548

Title: Development of a genosensor for peanut allergen ARA h 2 detection and its optimization by surface response methodology
Author(s): Sánchez-Paniagua López, Marta
Frutos Cabanillas, Gloria
Lobo Castañón, María Jesús
López Ruiz, Beatriz
Keywords: Electrochemical genosensor
Ara h2 peanut allergen
Issue date: 2014
Publisher: Elsevier
Publisher version: http://dx.doi.org/10.1016/j.bios.2014.06.065
Citation: Biosensors and Bioelectronics, 62, p. 350-356 (2014); doi:10.1016/j.bios.2014.06.065
Format extent: p. 350-356
Abstract: A new selective electrochemical genosensor has been developed for the detection of an 86-mer DNA peanut sequence encoding part of the allergen Arah2(conglutin-homologprotein).The method is base on a sandwich format,which presents two advantages:it permits shortening the capture probe and avoids labeling of the target.Screen-printed gold electrodes have been used as platformfor the immobilization of oligo nucleotides by the well-knownS–Aubond.Mixedself-assembled monolayers (SAM), including thiol-modified capture probe and mercaptohexanol,were prepared to a chieve an organized, homogeneous and not too compact SAM in which unspecific adsorption of the capture probe would be prevented...
URI: http://hdl.handle.net/10651/32548
ISSN: 0956-5663
Sponsored: This work has been cofinancied by Project MINECO-13-CTQ- 12-31157 and Project MICINN-08-CTQ-2008-02429
Project id.: MEC/MINECO-13-CTQ- 12-31157
MEC/MICINN-08-CTQ-2008-02429
Appears in Collections:Química Física y Analítica
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