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Repositorio de la Universidad de Oviedo. > Producción Bibliográfica de UniOvi: RECOPILA > Artículos >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10651/32546

Title: Affinity of aptamers binding 33-mer gliadin peptide and gluten proteins: Influence of immobilization and labeling tags
Author(s): Amaya González, Sonia
López López, Laura
Miranda Castro, Rebeca
Santos Álvarez, Noemí de los
Miranda Ordieres, Arturo José
Lobo Castañón, María Jesús
Keywords: Aptamer
Gluten
Binding constant
Issue date: 2015
Publisher: Elsevier
Publisher version: http://dx.doi.org/10.1016/j.aca.2015.02.053
Citation: Analytica Chimica Acta, 873, p. 63-70 (2015); doi:10.1016/j.aca.2015.02.053
Format extent: p. 63-70
Abstract: Aptamers are starting to increase the reagents tool box to develop more sensitive and reliable methods for food allergens. In most of these assays, aptamers have to be modified for detection and/or immobilization purposes. To take full advantage of their affinity, which decisively influence the detectability, these modifications must be faced rationally. In this work, a recently developed aptamer for an immunotoxic peptide of gliadin associated to celiac disease is used in different configurations and modified with various markers and anchored groups to evaluate the influence of such modifications on the real affinity...
URI: http://hdl.handle.net/10651/32546
ISSN: 0003-2670
1873-4324
Sponsored: This work was financed by Spanish Government Project CTQ2012-31157, and the European Regional Development Fund.
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